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To understand the inflammatory microenvironment and microbiome factors Synthetic Steroids SARMs are synthetic chemicals designed to mimic the effects of testosterone and other anabolic steroids. SARMs have been used as "testicles" to enhance athletic performance in humans for thousands of years, but their effects on the human body in particular are not fully understood. The main role of SARMs in the body is as growth promoters (i.e., increasing the number and length of fibrous tissue in the body), which can result in tissue growth and/or the formation of tumors. However, despite its important role in tissue growth, little is known about their role or the effects of SARMs on any important immune system organ. SARMs may alter the inflammatory process in the body; however, the specific impact of SARMs on these processes is not known. The main inflammation mechanisms by which SARMs can affect immune system function are: 1) Increased intracellular ROS in the host cell, which can alter the signaling of specific genes resulting in increased mRNA (or protein) messenger RNA. When cellular ROS exceeds the threshold needed to drive transcription of pro-inflammatory genes, such as tumor necrosis factor alpha (TNFα) or interleukin 6 (IL-6) or the cytokine interferon-γ (IFN-γ), the inflammatory response causes cell death, and the host cell stops responding to signals for the growth of tumor cells. Increased NF-κB activation also occurs in this pathway when cellular ROS exceeds the threshold needed to drive transcription of pro-inflammatory genes. 2) Increased production of reactive oxygen species (ROS), an oxidative stress mechanism, in the cell. This results in a variety of events, including the induction of the transcription of pro-inflammatory genes. The inflammatory microenvironment by which SARMs can affect immune function is unknown since it is unclear how the microenvironment of tissue changes following SARMs administration. In rodents, the tissue microenvironment is changed upon administration of testosterone and other SARMs. As demonstrated by our human study, the level of neutrophils, monocytes, and lymphocytes increased after treatment of prostate cells with 1,5-methyl-4-isoxazolepropionic acid (DMPA) (11) and testosterone (12), and serum concentrations of TNFα, IL-6, and MCP-1 were higher than those without testosterone in the prostate tissue from men treated with DMPA (12). Further, 1,5-methyl-4-isoxazolepropionic acid induces expression of the inflammatory cytokine MCP-1 in cultured monocytes via up-regulation of genes encoding TNFα and IκB Related Article: